Cost Reduction of PCR Thermal Cycler
نویسندگان
چکیده
This paper presents a PC-based thermal cycler with a local-host architecture, where a PC is employed as the host. The local system only executes the thermal cycling process and the PC is responsible for the management of PCR protocols with a convenient GUI. The presented local-host system greatly reduces the development time and the maintenance cost, providing an economical PCR thermal cycler.
منابع مشابه
PC-Based PCR Thermal Cycler
This paper presents a PC-based PCR thermal cycler with a host-local architecture, where a PC is employed as the host. The local system only executes the thermal cycling process and the PC is responsible for the management of PCR protocols with a convenient GUI. The presented host-local system greatly reduces the development time and the maintenance cost, providing an economical PCR thermal cycler.
متن کاملA Rapid and Low-Cost PCR Thermal Cycler for Low Resource Settings
BACKGROUND Many modern molecular diagnostic assays targeting nucleic acids are typically confined to developed countries or to the national reference laboratories of developing-world countries. The ability to make technologies for the rapid diagnosis of infectious diseases broadly available in a portable, low-cost format would mark a revolutionary step forward in global health. Many molecular a...
متن کاملInterlaboratory study on thermal cycler performance in controlled PCR and random amplified polymorphic DNA analyses.
BACKGROUND Intercomparisons of PCR-based data between laboratories require an assurance of assay reproducibility. We performed an interlaboratory study to investigate the contribution made by a variety of thermal cyclers to PCR performance as measured by interblock reproducibility and intrablock repeatability. METHODS Two standardized assays designed to minimize the introduction of non-therma...
متن کاملEvaluation of a rapid air thermal cycler for detection of Mycobacterium tuberculosis.
The Air Thermal Cycler (ATC) (Idaho Technology, Idaho Falls, Idaho) utilizes the unique technology of small-volume glass capillary tubes and high-velocity air for the heating and cooling medium for the PCR. Standard heat block thermal cycler (HBTC) and ATC performance characteristics were compared for the detection of Mycobacterium tuberculosis. Sensitivity was 100% for all smear-positive, M. t...
متن کاملLow-Cost Temperature Logger for a Polymerase Chain Reaction Thermal Cycler
Polymerase chain reaction (PCR) is a method of amplifying DNA which is normally carried out with a thermal cycler. To obtain more accurate and reliable PCR results, the temperature change within the chamber of the thermal cycler needs to be verified and calibrated regularly. Commercially available temperature loggers commonly used for temperature verification tests usually require a graphical u...
متن کامل